In Vivo Real-time Monitoring and Evaluation for Super- oxide Anion Radical Generation with an Electrochemical Sensor

Recently, we established an in vivo method to directly and continuously monitor and evaluate O2 using an electrochemical O2 sensor. The generated O2 is measured as a current and evaluated as a difference in the current from the baseline to the actual reacted O2 current (ΔI) and a quantified partial value of electricity (Q), which is calculated by integration of differences between baseline and actual reacted current. To clarify the dynamics of O2 in vivo and their O2-related pathophysiology, the accuracy and efficacy of this method were confirmed in phosphate-buffered saline and human blood and we applied this sensor to rat models of endotoxemia, forebrain ischemia-reperfusion (FBI/R), and heatstroke. This is a novel method for measuring O2 in vivo, and could be used to monitor and treat the pathophysiology caused by excessive O2 generation in animals and humans.